mtorc2 inhibitor jr ab2  (MedChemExpress)

Journal: Cell Death & Disease

Article Title: miR-582 negatively regulates pre-B cell proliferation and survival through targeting Hif1α and Rictor

doi: 10.1038/s41419-022-04560-y

Figure Lengend Snippet: A Pre-B cells were infected with anti-miR-582 lentivirus and cultured for 72 h. The level of p-AKT, AKT, p-FoxO1, and FoxO1 was determined by Western blotting with β-actin as a reference control ( n = 4–5). B Pre-B cells were infected with anti-miR-582 lentivirus and cultured in the presence of the mTORC2 inhibitor JR-AB2-011 for 72 h. The level of p-AKT, AKT, p-FoxO1, and FoxO1 was determined by Western blotting with β-actin as a reference control ( n = 4). C Pre-B cells were infected with anti-miR-582 lentivirus and cultured in the presence of the mTORC2 inhibitor JR-AB2-011 for 72 h. Cell proliferation and apoptosis were evaluated by CFSE labeling and Annexin V + and 7-AAD + , respectively, followed by flow cytometry, and quantitatively compared ( n = 3). D The signaling pathway of miR-582 regulate the proliferation and survival of pre-B cell. Bars represent means ± SEM, * P < 0.05, ** P < 0.01.

Article Snippet: In some experiments, Hif1α inhibitor Echinomycin (2 nM, Abcam, Cambridge, MA, USA) or mTORC2 inhibitor JR-AB2-011 (1 μM, MedChemexpress, Shanghai, China) were added in culture.

Techniques: Infection, Cell Culture, Western Blot, Control, Labeling, Flow Cytometry